Review



pcdna4 0 vector  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    New England Biolabs pcdna4 0 vector
    Pcdna4 0 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna4+0+vector/NotI/pmc12400676-133-9-29
    Average 99 stars, based on 5599 article reviews
    pcdna4 0 vector - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging
    Article Snippet: Using the amplicons of NMJSRV-F/NMJSRV-R and 3′RACE-F/3′RACE-R as templates, PCR amplification was performed with the Q5® High-Fidelity DNA Polymerase Kit (New England Biolabs, NEB) and the primer pairs pc-NMJS12- KpnI -F/U3u-NMJS12 R, U3d-F/U3d-U5-R, and U3-U5-F/pc-U5- NotI -R to generate the corresponding target fragments. .. Following amplification, the three fragments were cloned into the pcDNA4.0 vector (digested with KpnI and NotI and containing the CMV strong promoter) via fusion PCR and T4 DNA ligase (New England Biolabs, NEB). ..

    Clone Assay:

    Article Title: Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging
    Article Snippet: Using the amplicons of NMJSRV-F/NMJSRV-R and 3′RACE-F/3′RACE-R as templates, PCR amplification was performed with the Q5® High-Fidelity DNA Polymerase Kit (New England Biolabs, NEB) and the primer pairs pc-NMJS12- KpnI -F/U3u-NMJS12 R, U3d-F/U3d-U5-R, and U3-U5-F/pc-U5- NotI -R to generate the corresponding target fragments. .. Following amplification, the three fragments were cloned into the pcDNA4.0 vector (digested with KpnI and NotI and containing the CMV strong promoter) via fusion PCR and T4 DNA ligase (New England Biolabs, NEB). ..

    Article Title: Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1.
    Article Snippet: .. The Flag-tagged ZIKV NS5 fragment was digested and cloned into the KpnI- and XhoI-cut pcDNA4.0 vector using T4 DNA ligase (NEB, MA, USA). ..

    Article Title: Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1
    Article Snippet: .. The Flag-tagged ZIKV NS5 fragment was digested and cloned into the Kpn I- and Xho I-cut pcDNA4.0 vector using T4 DNA ligase (NEB, MA, USA). ..

    Plasmid Preparation:

    Article Title: Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging
    Article Snippet: Using the amplicons of NMJSRV-F/NMJSRV-R and 3′RACE-F/3′RACE-R as templates, PCR amplification was performed with the Q5® High-Fidelity DNA Polymerase Kit (New England Biolabs, NEB) and the primer pairs pc-NMJS12- KpnI -F/U3u-NMJS12 R, U3d-F/U3d-U5-R, and U3-U5-F/pc-U5- NotI -R to generate the corresponding target fragments. .. Following amplification, the three fragments were cloned into the pcDNA4.0 vector (digested with KpnI and NotI and containing the CMV strong promoter) via fusion PCR and T4 DNA ligase (New England Biolabs, NEB). ..

    Polymerase Chain Reaction:

    Article Title: Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging
    Article Snippet: Using the amplicons of NMJSRV-F/NMJSRV-R and 3′RACE-F/3′RACE-R as templates, PCR amplification was performed with the Q5® High-Fidelity DNA Polymerase Kit (New England Biolabs, NEB) and the primer pairs pc-NMJS12- KpnI -F/U3u-NMJS12 R, U3d-F/U3d-U5-R, and U3-U5-F/pc-U5- NotI -R to generate the corresponding target fragments. .. Following amplification, the three fragments were cloned into the pcDNA4.0 vector (digested with KpnI and NotI and containing the CMV strong promoter) via fusion PCR and T4 DNA ligase (New England Biolabs, NEB). ..



    Similar Products

    99
    New England Biolabs pcdna4 0 vector
    Pcdna4 0 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna4+0+vector/NotI/pmc12400676-133-9-29
    Average 99 stars, based on 1 article reviews
    pcdna4 0 vector - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs pcdna4 0 sars cov 2 frameshift reporter vector
    Pcdna4 0 Sars Cov 2 Frameshift Reporter Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna4+0+vector/XhoI/pm40514371-295-4-14
    Average 99 stars, based on 1 article reviews
    pcdna4 0 sars cov 2 frameshift reporter vector - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Thermo Fisher pcdna4 0 myc hisa vector
    Pcdna4 0 Myc Hisa Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna4+0+vector/pm37434327-44-21-23
    Average 86 stars, based on 1 article reviews
    pcdna4 0 myc hisa vector - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher pcdna4 0 vector
    HTR8/SVneo cells were transfected with miR-195 and ActRIIA alone or in combination, with scramble siRNA (NC) or <t>pcDNA4</t> vector (pcDNA4) as corresponding negative control. Transwell insert assay was performed to monitor cell invasiveness (A) and Gelatin zymography was performed to measure MMP-2 and MMP-9 productions (B). The statistical analysis was carried out by ANOVA according to three independent experiments, and the values were presented as Mean±SEM. *, compared with corresponding group as indicated, p <0.05.
    Pcdna4 0 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna4+0+vector/pmc03378540-70-17-19
    Average 86 stars, based on 1 article reviews
    pcdna4 0 vector - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher mammalian expression vector pcdna4 0 to
    HTR8/SVneo cells were transfected with miR-195 and ActRIIA alone or in combination, with scramble siRNA (NC) or <t>pcDNA4</t> vector (pcDNA4) as corresponding negative control. Transwell insert assay was performed to monitor cell invasiveness (A) and Gelatin zymography was performed to measure MMP-2 and MMP-9 productions (B). The statistical analysis was carried out by ANOVA according to three independent experiments, and the values were presented as Mean±SEM. *, compared with corresponding group as indicated, p <0.05.
    Mammalian Expression Vector Pcdna4 0 To, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna4+0+vector/pmc03541790-149-6-10
    Average 86 stars, based on 1 article reviews
    mammalian expression vector pcdna4 0 to - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    HTR8/SVneo cells were transfected with miR-195 and ActRIIA alone or in combination, with scramble siRNA (NC) or pcDNA4 vector (pcDNA4) as corresponding negative control. Transwell insert assay was performed to monitor cell invasiveness (A) and Gelatin zymography was performed to measure MMP-2 and MMP-9 productions (B). The statistical analysis was carried out by ANOVA according to three independent experiments, and the values were presented as Mean±SEM. *, compared with corresponding group as indicated, p <0.05.

    Journal: PLoS ONE

    Article Title: Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression

    doi: 10.1371/journal.pone.0038875

    Figure Lengend Snippet: HTR8/SVneo cells were transfected with miR-195 and ActRIIA alone or in combination, with scramble siRNA (NC) or pcDNA4 vector (pcDNA4) as corresponding negative control. Transwell insert assay was performed to monitor cell invasiveness (A) and Gelatin zymography was performed to measure MMP-2 and MMP-9 productions (B). The statistical analysis was carried out by ANOVA according to three independent experiments, and the values were presented as Mean±SEM. *, compared with corresponding group as indicated, p <0.05.

    Article Snippet: To construct the ActRIIA expressing plasmid (pcDNA4-ActRIIA), the coding sequence of ActRIIA was amplified and inserted into pcDNA4.0 vector (Invitrogen, Carlsbad, CA) at the EcoRI and HindIII restriction sites.

    Techniques: Transfection, Plasmid Preparation, Negative Control, Zymography