pcdna4 0 vector (New England Biolabs)
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Pcdna4 0 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna4+0+vector/NotI/pmc12400676-133-9-29
Average 99 stars, based on 5599 article reviews
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Amplification:Article Title: Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging Article Snippet: Using the amplicons of NMJSRV-F/NMJSRV-R and 3′RACE-F/3′RACE-R as templates, PCR amplification was performed with the Q5® High-Fidelity DNA Polymerase Kit (New England Biolabs, NEB) and the primer pairs pc-NMJS12- KpnI -F/U3u-NMJS12 R, U3d-F/U3d-U5-R, and U3-U5-F/pc-U5- NotI -R to generate the corresponding target fragments. .. Following amplification, the three fragments were cloned into the Clone Assay:Article Title: Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging Article Snippet: Using the amplicons of NMJSRV-F/NMJSRV-R and 3′RACE-F/3′RACE-R as templates, PCR amplification was performed with the Q5® High-Fidelity DNA Polymerase Kit (New England Biolabs, NEB) and the primer pairs pc-NMJS12- KpnI -F/U3u-NMJS12 R, U3d-F/U3d-U5-R, and U3-U5-F/pc-U5- NotI -R to generate the corresponding target fragments. .. Following amplification, the three fragments were cloned into the Article Title: Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1. Article Snippet: .. The Flag-tagged ZIKV NS5 fragment was digested and cloned into the KpnI- and Article Title: Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1 Article Snippet: .. The Flag-tagged ZIKV NS5 fragment was digested and cloned into the Kpn I- and Xho I-cut Plasmid Preparation:Article Title: Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging Article Snippet: Using the amplicons of NMJSRV-F/NMJSRV-R and 3′RACE-F/3′RACE-R as templates, PCR amplification was performed with the Q5® High-Fidelity DNA Polymerase Kit (New England Biolabs, NEB) and the primer pairs pc-NMJS12- KpnI -F/U3u-NMJS12 R, U3d-F/U3d-U5-R, and U3-U5-F/pc-U5- NotI -R to generate the corresponding target fragments. .. Following amplification, the three fragments were cloned into the Polymerase Chain Reaction:Article Title: Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging Article Snippet: Using the amplicons of NMJSRV-F/NMJSRV-R and 3′RACE-F/3′RACE-R as templates, PCR amplification was performed with the Q5® High-Fidelity DNA Polymerase Kit (New England Biolabs, NEB) and the primer pairs pc-NMJS12- KpnI -F/U3u-NMJS12 R, U3d-F/U3d-U5-R, and U3-U5-F/pc-U5- NotI -R to generate the corresponding target fragments. .. Following amplification, the three fragments were cloned into the |
